In vitro seeds germination and plantlets development of Gram | 16093
International Research Journals

International Research Journal of Plant Science

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In vitro seeds germination and plantlets development of Grammatophyllum scriptum Lindl. (Orchidaceae)


Barahima Abbas*, Florentina Heningtyas Listyorini, and Besse Amriati

Many species of Orchidaceae in Papua (Indonesia territorial) grow naturally in the forest and one of interesting orchid is Grammatophyllum scriptum. Seeds of G. scriptum were germinated successfully and grew well in the asymbiotic culture. The best medium were observed for germinating the G. scriptum seeds, viz. Knudson-C (KnC) basal medium supplemented with 30% (v/v) maturity coconut water/milk (CW). All the seeds were observed those overall germination, protocoms formation and seedling development (about 100%). The best medium for growing and development of seedlings to become fully expanded plantlets was determined half strength of Murashige and Skoog (1/2MS) medium supplemented with 40% (v/v) CW. The plantlets of G. scriptum were resulted from these research approximately more than 2,000 individuals.

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