Estimation of proteins profile by gel electrophoresis (SDS-P | 73801
International Research Journals

International Research Journal of Plant Science

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Estimation of proteins profile by gel electrophoresis (SDS-PAGE) in citrullus colocynthis (Linn.) schrad. in vivo and in vitro


Dr. Mahesh Chand Meena*

Proteins form the class of biological macromolecules that have the most well defined physico-chemical properties and consequently they are easier to isolate and characterize than nucleic acids. Gel electrophoresis is a useful tool for characterizations of plant proteins. The recognitions of protein using electrophoresis is necessary and valuable for taxonomic studies in plants, animals, microorganisms and the viroids. Differential banding pattern for protein in Citrullus Colocynthis (Linn.) Schrad. in all in vivo (leaf, stem, fruit and root) and in vitro (callus and differentiating callus) samples was observed. In vivo samples (leaf, stem, fruit and root) showed three bands while in vitro (callus and differentiating callus) samples showed five bands. Variation in intensity of protein bands was also observed in all samples. Fruit and in vitro samples showed more intense banding pattern. Rm value of first upper band showed marginal variation in all samples (leaf, stem, fruit, root, callus and differentiating callus).

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