Escherichia Coli Molecular Characterization and Antimicrobia | 90727
International Research Journals

International Research Journal of Biochemistry and Bioinformatics

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Escherichia Coli Molecular Characterization and Antimicrobial Susceptibility Associated with Diarrhea among Children in Babil Province: Genotype, Phenotype, and Virus Genes Markers


Scarlett Williams*

Escherichia coli (DEC) strains that cause diarrhoea in children have been identified and classified in comparatively few investigations in Babil. This study looked on DEC among Babil children who were hospitalized and under the age of two. Using culture on MacConkey and EMB agar, the faeces of 200 children with diarrhea and 75 children without diarrhea were analyzed, and the E. coli isolates were checked for the presence of diarrheagenic E (Kaper JB et al., 2004). coli types and antibiotic susceptibility patterns. PCR for the detection of virulence genes. The detection of 10 different virulence genes for five different pathogroups of DEC, including enteroaggregative (EAEC), enteropathogenic (EPEC), enterotoxigenic (ETEC), enteroinvasive (EIEC), and enterohemorghagic Escherichia coli, was done using a multiplex PCR system approach (EHEC). With typical EAEC and typical EPEC predominating, this investigation showed a significant prevalence of antibiotic resistance among normal intestinal E. coli isolates as well as a high incidence of diarrhea genic E. coli isolates (Aranda KRS et al., 2004). The sensitivity for detecting ETEC strains in hospitalized children can be determined by using primers for both ST variants.

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