Citrus hytrix (Rutaceae) is well known as Kaffir lime was found to be beneficial as essential oil – perfumery. Ethanolic extracts of Citrus hystrix leaves revealed the presence of phytochemical constituents viz., Saponins, Tannins, Lipids, Flavonoids and Terpenoids. The objective of the present study is to determine the efficacy of ethanolic extracts of Citrus hystrix for bactericidal effects against MHSA and also analyzed for antioxidant potentials. Ethanolic extract of Citrus hystrix has been used for estimation of bactericidal effects against Methicillin Resistance Staphylococcus aureus at various eluted fractionated compounds of Citrus hystrix. The peptides possess the high activity subjected for HPTLC fingerprinting analysis. DPPH analysis were also performed to assess the free radical scavenging activity. Antioxidant activity displayed superior activity with an IC50 of 2.8μg/ml respectively. Fractions (EF13, EF14, EF15) showed high bactericidal activity against MRSA. Furthermore, HPTLC of the fractionated compound indicate the presence of biological active products - Quercetin, Rutin and Gallic acid with high area. This study suggests that, ethanolic extracts of Citrus hystrix leaves exhibits antibacterial activity against Methicillin resistance Staphylococcus aureus. Phytomedicines are now an important and beneficial zone of the recommended treatment.
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