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International Research Journal of Plant Science

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Effect of media type and explant source on micropropagation of Dalbergia sissoo Roxb. - An important multipurpose forest tree

Abstract

Thirunavoukkarasu M, Panda PK, Nayak P, Behera PR, Satpathy GB

Attempts were made to evaluate the suitable explant and media type for micropropagation of Dalbergia sissoo from axillary bud explants of epicormic and coppice shoots origin. Murashige and Skoog (MS) medium and Woody Plant medium (WP) supplemented with N6 –benzyl adenine (BA) either alone or in combination with indole-3-acetic acid (IAA) were used for plantlet production. Auxilary buds of coppice shoots origin responded better than of epicormic shoot origin. Thus, the axillary buds of coppice shoot origin exhibited maximum response (84.6 %) on the medium with MS + 6.6 µM BA + 1.14 µM IAA where an average of 8.38 shoots/explant with 5.87 cm shoot length/shoot was recorded. MS medium was found to be superior to WP medium in terms of shoot numbers and shoot length. Rooting of in vitro shootlets was achieved on half strength MS medium supplemented with either IAA or indole-3- butyricacid (IBA). Rooting was best in the medium supplemented with 7.5 µM IBA. Rooted plantlets were acclimatized with 70 % survival rate.

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