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International Research Journal of Microbiology

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Dilute acid pretreatment of millet and guineacorn husks for bioethanol production

Abstract

Rabah, A. B.*, Oyeleke, S. B., Manga, S. B. and Hassan, L. G.

Millet and guineacorn husks were pretreated with 3 %, 4 % and 5 % hydrochloric acid concentrations at 30 oC, 40 oC and 50 oC for 20, 25 and 30 minutes prior to bioethanol production over a seven day period using Saccharomyces cerevisiae, Zymomonas mobilis and the combination of the two organisms as the fermentative organisms. High reducing sugar yields (4.6 %) were obtained from millet husk hydrolysates at all the temperature regimes used (30 oC, 40 oC and 50 oC), most importantly at 4 % acid concentration. Also, the result revealed that the combination of S. cerevisiae and Z. mobilis produced a yield of 0.44 % after the seventh day period of fermentation from guineacorn husk. This indicated that dilute acid pretreatment could be a suitable option for freeing fermentable sugars from lignocellulosic wastes.

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