Bioactive glass with antimicrobial agents: In vitro evaluati | 17082
International Research Journals

Bioactive glass with antimicrobial agents: In vitro evaluation


Monize F. F. De Carvalho, Rosana Z. D. Fernandes, Ângela L. Andrade, Vagner R. Santos, Karine A. T. Tavano, Walison A. Vasconcelos

Bioactive glass is biocompatible and has the abilit y to stimulate bone formation. The aim of the present study was to evaluate the efficiency of bio active glass in releasing tetracycline and/or hydrocortisone through an in vitro growth inhibition study involving microorganisms c ommonly found in the oral cavity: Staphylococcus aureus (ATCC 27664), Enterococcus faecalis (ATCC 12399) and Streptococcus mutans (ATCC 70069). The bioactive glass was prepared usin g the sol-gel method and had a molar composition of 80% SiO2, 4% P2O5 and 16% CaO. Four groups were formed: a drug-free control group (CG); a hydrocort isone group (HG) with 2% (in weight) of the drug incorporated into the bioactive glass; a tetracycli ne group (TG) containing 2% (in weight) of the drug ; and a hydrocortisone-tetracycline group (HTG) conta ining 2% of each drug. Test specimens were distributed in Petri dishes containing agar previou sly inoculated with the microorganisms and incubated for 24 hours. Statistical analysis involv ed the Kruskal-Wallis and Mann-Whitney tests. The CG and HG proved ineffective in inhibiting the grow th of the microorganisms. The TG exhibited inhibition zones on all microorganisms tested. The H TG had the largest inhibition zones for Staphylococcus aureus (ATCC 27664) and Enterococcus faecalis (ATCC 12399), with statistically significant differences in comparison to the other groups (p < 0.001). The present study demonstrates that bioactive glass is able to releas e tetracycline for effective antibiotic action.

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