Monize F. F. De Carvalho, Rosana Z. D. Fernandes, ÃÆÃângela L. Andrade, Vagner R. Santos, Karine A. T. Tavano, Walison A. Vasconcelos
Bioactive glass is biocompatible and has the abilit y to stimulate bone formation. The aim of the present study was to evaluate the efficiency of bio active glass in releasing tetracycline and/or hydrocortisone through an in vitro growth inhibition study involving microorganisms c ommonly found in the oral cavity: Staphylococcus aureus (ATCC 27664), Enterococcus faecalis (ATCC 12399) and Streptococcus mutans (ATCC 70069). The bioactive glass was prepared usin g the sol-gel method and had a molar composition of 80% SiO2, 4% P2O5 and 16% CaO. Four groups were formed: a drug-free control group (CG); a hydrocort isone group (HG) with 2% (in weight) of the drug incorporated into the bioactive glass; a tetracycli ne group (TG) containing 2% (in weight) of the drug ; and a hydrocortisone-tetracycline group (HTG) conta ining 2% of each drug. Test specimens were distributed in Petri dishes containing agar previou sly inoculated with the microorganisms and incubated for 24 hours. Statistical analysis involv ed the Kruskal-Wallis and Mann-Whitney tests. The CG and HG proved ineffective in inhibiting the grow th of the microorganisms. The TG exhibited inhibition zones on all microorganisms tested. The H TG had the largest inhibition zones for Staphylococcus aureus (ATCC 27664) and Enterococcus faecalis (ATCC 12399), with statistically significant differences in comparison to the other groups (p < 0.001). The present study demonstrates that bioactive glass is able to releas e tetracycline for effective antibiotic action.
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