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Antimicrobial evaluation and acute and sub-acute toxicity studies on a commercial polyherbal formulation, ├ó┬?┬?Ade and Ade Antidiabetic├?┬«├ó┬?┬Ł used in the treatment of diabetes in southwestern Nigerian| Abstract

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Antimicrobial evaluation and acute and sub-acute toxicity studies on a commercial polyherbal formulation, ├ó┬?┬?Ade & Ade Antidiabetic├?┬«├ó┬?┬Ł used in the treatment of diabetes in southwestern Nigerian

Abstract

Ogbonnia SO, Mbaka GO, Emordi J., Nkemhule F., Joshua P, Usman A, Odusanya P, Ota D

This study evaluated the microbial purity, acute an d sub-chronic toxicities in rodents of a polyherbal formulation, Ade & Ade Antidiabetic®, an antidiabet ic formulation, prepared with Ocimum gratissimum, Citrullus lanatus, Momordica charantia, Chrysophyll um delevoyi and Uncaria tomentosa leaves. Microbial purity was evaluated on some bacterial and fungal organisms using appropriate di agnostic media. Acute toxicity of the polyherbal formulation was evaluated in Swiss albino mice by administering orally graded doses of the lyophilized formulation in the ranges o f 1.0 g to 20.0 g/kg body weight to the animals and observed continuously for the first 4 hours and hourly for t he next 12 hours then 6 hourly for 56 hours (72 hr). Wistar rats wer e also fed with different doses of the lyophilized formulation for 30 days and the effects on the body weight, biochemical and hematological profiles and tissue histology were evaluated (sub-acute toxicit y model). The presence of E.coli bacterial organisms with loads above officially accepted limit were obs erved in the formulation. The median acute toxicity value (LD 50 ) of the polyherbal formulation was determined to be 15.0 g/kg body wei ght. Significant increase in the body weight was observed in all the treated compared to the control. Significant increase (p ≤ 0.05) in creatinine level occurred while aspartate aminotransferases (AST) and alanine aminotransferases (ALT) levels showed marked decrea se. Total cholesterol (TC) and low density lipoprotein (LDL)-cholesterol levels decreased appr eciably while triglyceride (TG) and high density lipoprotein (HDL)-cholesterol levels exhibited mark ed increase. The extract significantly increased (p<0.05) hemoglobin and RBC contents in all the tre ated groups compared to the control. There was also an increase in WBC which was complemented by a n increase in lymphocyte cells.

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