Antimicrobial activity of soil actinomycetes Isolated from A | 17646
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International Research Journal of Microbiology

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Antimicrobial activity of soil actinomycetes Isolated from Alkharj, KSA


Muharram, M M; Abdelkader, M S; Alqasoumi, S I

Thirty three soil-isolates of actinomycetes were screened for their antimicrobial activities. Three potent isolates, (SA110, SC110 and SF202), showed antibacterial and antifungal activity against one or more strain of Escherichia coli, ATCC 8739; Candida albicans ATCC 66027; Candida albicans ATCC 2091, Aspergillus niger, ATTC 16404 Staphylococcus aureus, ATCC 29213; Bacillus subtilis, ATCC 11774 and Streptococcus epidermidis ATCC 12228. Screening of the antimicrobial activity was conducted by Cross-streak, Cork-borer and agar well methods. The isolates of SA110, SC110 and SF202 were identified as Streptomyces samposonii, Streptomyces albidoflavus and Streptomyces roche, respectively based on a variety of morphological, physiological and molecular analysis of 16S rDNA gene sequence isolates. Factors affecting the biosynthesis of antimicrobial agent like different inoculum size, pH values, temperatures, incubation period, and different carbon and nitrogen sources were analyzed.

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