Shinkafi, S. A.
Antidermatophytic activities, phytochemical screenings and chromatographic studies of Pergularia tomentosa and Mitracarpus Scaber (leaves) were determined. The extracts of the plants were prepared at five different concentrations (10, 20, 40, 80 and 160 mg/ml). The results of antidermatophytic activities of crude extracts of the two plants exhibited promising antidermatophytic activites against Trichophyton rubrum and Trichophyton mentagrophyte at concentration of 10 mg/ml. Growth of Microsporum gypseum was controlled at 40 mg/ml by both plants. Similarly, the results for phytochemical screening showed the presence of Tannins, Flavonoids, Alkaloids, Saponins, Glycosides, Saponinglycosides, Cardiacglycosides, Anthraquinones and Steroids. Saponins and Flavonoids of both plants were present in large quantities. Column chromatographic fractionation of P. tomentosa and M. scaber was conducted using extracts of various organic solvents. Assessment of each fraction for antidermatophytic activity revealed chloroform fraction 4 (CHL4) of P. tomentosa and chloroform fraction 1 (CHL1) of M. scaber as the most active fractions against Trichophyton rubrum, Trichophyton mentagrophyte and Microsporum gypseum at 10 mg/ml. MIC and MFC determinations of the active column fractions produced inhibitory action on T. rubrum, T. mentagrophyte and M. gypseum at 10 mg/ml each. From the results obtained it can be concluded that the presence of the above phytochemical compounds in P. tomentosa and M. scaber may be responsible for the antidermatophytic activities exhibited by the plants against most of the dermatophytes tested.
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