The Comparison of Antioxidative and Proliferation Inhibitor | 16691
International Research Journals

International Research Journal of Biochemistry and Bioinformatics

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The Comparison of Antioxidative and Proliferation Inhibitor Properties of Piper betle L., Catharanthus roseus [L] G.Don, Dendrophtoe petandra L., Curcuma mangga Val. Extracts on T47D Cancer Cell Line


Wahyu Widowati, Tjandrawati Mozef, Chandra Risdian, Hana Ratnawati, Susy Tjahjani, Ferry Sandra.

Breast cancer is the most common cancer among women. It is estimated that one of eight women will be diagnosed with breast cancer in women. The betel leaves (Piper betle L.), madagascar prewinkle (Catharanthus roseus [L] G.Don), mango parasite (Dendropthoe petandra L.) and white saffron (Curcuma mangga Val.) have been reported to exhibit antioxidant, and antimutation that suggested the chemopreventive potential against various cancer including breast cancer. This research was conducted to investigate anticancer activity of P. betle, C. roseus, D. petandra and C. mangga extracts on breast cancer cell line T47D, and antioxidant activity. The anticancer activity was determined with MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. The antioxidant activity was determined by using in vitro assay of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. P. betle , C. roseus extracts were able to inhibit T47D cell proliferation with IC50 55.2 �?¼g/ml, 26.22 �?¼g/ml and D. petandra, C. mangga extracts with IC50 728.05 �?¼g/ml, 404.76 �?¼g/ml, while DPPH scavenging activity (IC50) on P. betle, C. roseus, D. petandra, C. mangga extracts were respectively 5.49 �?¼g/ml, 102.96 �?¼g/ml, 4.74 �?¼g/ml, 277.79 �?¼g/ml. P. betle and D. petandra extracts are more active antioxidant compared to C. roseus and C. mangga.

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