Sterilization of immature blowflies (Calliphoridae) for use | 17894
International Research Journals

Sterilization of immature blowflies (Calliphoridae) for use in larval therapy


Patricia Jacqueline Thyssen, Mariana Prado Nassu, Maria José Trevizani Nitsche, Domingos da Silva Leite

The larval therapy involves the intentional applica tion of sterilized and alive dipterans larvae on non - healing wounds with the purpose of cleaning out the necrotic tissue to facilitate the healing process. This study aimed to evaluate the sterilization proce ss and survival of Chrysomya megacephala (Fabricius), Chrysomya putoria (Wiedemann) and Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae) larvae, after treatment with sodium hypochlorite (NaClO). The experimental groups for each species were: control group (CG), in which it was used water; 0.5% sodium hypochlorite group (0.5% SHG); and 1% SHG. For testing the time for ea ch treatment, the eggs were washed for one or three minutes in the described solution. After, ali quots from the “rinsing water” were inoculated in Plate Count Agar (PCA) and blood agar (BA), with in cubation at 37ºC and readings performed after 24 and 48 hours for both. For all species, there were no bacterial growth on PCA and BA regarding to both treatments, 0.5% SHG and 1.0% SHG. The surviva l rate and intervals of immersion were similar among the CG and the different concentrations of Na ClO for all species. In conclusion, sterilization of blowflies` eggs made by NaClO is an efficient metho d to obtain the sterilized larvae for larval therapy .

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