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International Research Journal of Plant Science

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Molecular Cloning of Senescence-Related cDNA, OsRab7, from Thai Jasmine Rice (Oryza sativa L. cv. KDML 105)

Abstract

Sugunya Pitakrattananukool, Supranee Sitthiphrom, Robert W. Cutler, Somboon Anuntalabhochai

In this work we cloned an OsRab7 DNA sequence encoding a small GTP-binding protein from Thai jasmine rice (Oryza sativa L. cv. KDML 105). All of the characteristic motifs of the small GTP-binding Rab family proteins were present in the OsRab7 sequence. The expression analysis revealed that this cDNA was present in various rice tissues including roots, young leaves, senescing leaves, flowers and seeds. In addition, the cDNA was also up-regulated under the following induced-senescence conditions, abscisic acid (ABA), ethylene, NaCl, dark treatment and wounding, suggesting that OsRab7 acted as a stress-inducible gene involved in the senescence process in Thai jasmine rice.

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