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International Research Journal of Microbiology

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Genomic Studies and genetic characterization of equid herpesvirus type 1 (EHV-1) strains: estimating the global similarity by distance methods.

Abstract

Giselle P. Martín Ocampos Claudio G. Barbeito , Cecilia M. Galos

Equid Herpesvirus type 1 (EHV-1) is a member of the genus Varicellovirus, which belongs to the subfamily Alphaherpesvirinae, family Herpesviridae. EHV-1 contains five non-codifying regions of variable sizes. One of them, called intergenic region (IR), is located between ORF 62 and ORF 63.This region was amplified by polymerase chain reaction and its products were sequenced. We analyzed 25 EHV-1 strains isolated from different geographical areas. Genomic analysis showed a high percentage of identity of the strains of the intergenic region. In 13 Argentinean strains, 18-bp insertions were found located in the 109168 bp position, whereas, in the AR6 strain, a 9-bp insert was found in the same position. The strains studied were classified using global similarity techniques and the analysis of genetic distances revealed that seven strains are grouped with foreign strains. Within this group a subgroup comprising an Argentinean and a British strain was identified. No relationship between the isolation years and the geographical origins of EHV-1 strains was found.

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