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International Research Journal of Microbiology

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Evaluation of Two Lactobacillus Strains as Probiotics with Emphasis in Utilizing Prebiotic Inulin as Energy Source

Abstract

Widodo, Nosa Septiana Anindita, Tiyas Tono Taufiq, and Tutik Dwi Wahyuningsih

The aim of this experiment was to examine the capability of two Lactobacillus strains as probiotics with emphasis in utilizing prebiotic inulin as energy source. Lactobacillus casei strain AP and AG were isolated from fecal of Indonesian infant, and had been reported in previous experiments. Characteristics as probiotics was evaluated in vitro based on the ability to grow on media at low pH and the addition up to 1.5% bile salts, the ability to inhibit the growth of Shigella flexnerii, Eschericia coli, Staphylococcus aureus and Enterococcus faecalis, the ability to attach gastric mucin, and the ability to consume prebiotic inulin as the only carbon source. Results showed that 80% of cells survived to pH 2.0 for 90 minutes, while survival of these two strains after growing at media supplemented with 1.5% bile salts was more than 70%. These two strains showed the ability to inhibit the growth of Shigella flexnerii, Eschericia coli, Staphylococcus aureus and Enterococcus faecalis. Further study on the adherence ability showed high level (70%) attachment on gastric mucin. Both strains were also able to grow normally using prebiotic inulin as the only carbon source. It was concluded that Lactobacillus casei strains AP and AG are potential candidates as probiotics and subject to further in vivo assessment.

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