Journal of Medicne and Medical Sciences (ISSN: 2141-9477) Vol. 4(11), pp. 423-432, November 2013. DOI: http:/dx.doi.org/10.14303/jmms.2013.138. Copyright © 2013 International Research Journals

 

Full Length Research Paper

Antimicrobial evaluation and acute and sub-acute toxicity studies on a commercial polyherbal formulation, Ade & Ade Antidiabetic®” used in the treatment of diabetes in southwestern Nigerian

1Ogbonnia SO, *2Mbaka GO, 3Emordi J., 1Nkemhule F., 1Joshua P, 4Usman A, 4Odusanya P, 5Ota D

1 Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria.

*2Department of Anatomy, Lagos State University College of Medicine, Ikeja, Lagos, Nigeria.

3Department of Pharmacology, College of Medicine, University of Lagos, Nigeria.

4Department of Pharmaceutical Technology and Pharmaceutical Microbiology, University of

Lagos, Nigeria.

5Department of Physiology, College of Medicine, University of Lagos, Nigeria.

*Correspondence to: Mbaka GO, email: mbaaka2gm@gmail.com

Received September 30, 2013; Accepted October 24, 2013

Abstract

 

This study evaluated the microbial purity, acute and sub-chronic toxicities in rodents of a polyherbal formulation, Ade & Ade Antidiabetic®, an antidiabetic formulation, prepared with Ocimum gratissimum, Citrullus lanatus, Momordica charantia, Chrysophyllum delevoyi and Uncaria tomentosa leaves. Microbial purity was evaluated on some bacterial and fungal organisms using appropriate diagnostic media. Acute toxicity of the polyherbal formulation was evaluated in Swiss albino mice by administering orally graded doses of the lyophilized formulation in the ranges of 1.0 g to 20.0 g/kg body weight to the animals and observed continuously for the first 4 hours and hourly for the next 12 hours then 6 hourly for 56 hours (72 hr). Wistar rats were also fed with different doses of the lyophilized formulation for 30 days and the effects on the body weight, biochemical and hematological profiles and tissue histology were evaluated  (sub-acute toxicity model). The presence of E.coli bacterial organisms with loads above officially accepted limit were observed in the formulation. The median acute toxicity value (LD50) of the polyherbal formulation was determined to be 15.0 g/kg body weight. Significant increase in the body weight was observed in all the treated compared to the control. Significant increase (p≤ 0.05) in creatinine level occurred while aspartate aminotransferases (AST) and alanine aminotransferases (ALT) levels showed marked decrease. Total cholesterol (TC) and low density lipoprotein (LDL)-cholesterol levels decreased appreciably while triglyceride (TG) and high density lipoprotein (HDL)-cholesterol levels exhibited marked increase. The extract significantly increased (p<0.05) hemoglobin and RBC contents in all the treated groups compared to the control. There was also an increase in WBC which was complemented by an increase in lymphocyte cells.

Keywords: Ocimum gratissimum, Citrullus lanatus, Momordica charantia, Chrysophyllum delevoyi and Uncaria tomentosa, acute, sub-acute toxicity.