Journal of Research in Environmental Science and Toxicology

Journal of Research in Environmental Science and Toxicology (ISSN: 2315-5698) Vol. 5(1) pp. 001-006,  February 2016. DOI: http:/ Copyright © 2016 International Research Journals


Full Length Research Paper

Estimation of fungicide toxicity for pathogen: Lasiodiplodia theobromae

*Adeniyi Dele Omoyele 

Plant Pathology Section, Cocoa Research Institute of Nigeria, P.M.B. 5244, Ibadan, Nigeria

*Corresponding author email:; +234(0)803-514-9863

Received November 15, 2015;  Accepted January 20, 2016



Lasiodiplodia theobromae attacks plants of major economic interest including cashew. This pathogen cause many problems to farmers resulting into heavy economic loss. Chemical control is currently one of the most effective ways to fight against this pathogen. The efficacy of sixfungicides: Mancozeb 80% WP, Copper-1-oxide 60% + metalaxyl M 6%, Copper hydroxide (57%), Cuprous oxide (86%), Carbendazim 50% WP and Copper-1-oxide 60% +metalaxyl 12% WP were tested, in-vitro on the phytopathogenic L. theobromae isolated from inflorescence dieback disease of cashew. The fungicides were tested at different concentrations: 2.5, 2.0, 1.5, 1.0, 0.5, 0.25 and 0.1mg/ml of the active ingredients. Toxicity responses were expressed as the effective concentration, which inhibits mycelial growth by 50%, (EC50). Of the six selected fungicides, carbendazim proved to be the most effective with EC50 value of the order of 0.068mg/ml followed by mancozeb with 0.21mg/ml and Copper-1-oxide 60% + metalaxyl M 6% with value of 0.67mg/ml. Other fungicides showed lower fungitoxicity with estimated EC50 values of 5.14 mg/ml, 6.66 mg/ml and 4.35mg/ml.


Keywords: Lasiodiplodia theobromae, cashew, fungicides, toxicology, active ingredients, mycelial inhibition, EC50



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