International Research Journal of Biotechnology

International Research Journal of Biotechnology Vol. 4(5), pp.89-93, May 2013         
Copyright © 2013 International Research Journals


Full Length Research Paper

In vitro sterilization protocol for micropropagation ofAchyranthes aspera L. node

1Monokesh Kumer Sen, *1,2Md. Mehedi Hassan,3Shamima Nasrin, 1M. Abu Hena Mostofa Jamal, 1A. N. M. Mamun-Or-Rashid, 1Biplab Kumar Dash

1Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh

2Department of Microbiology and Genetics and Institute for Medical Science, Chonbuk National University Medical School, Jeonju 561-712, South Korea

3Scientific Officer, Plant Biotechnology Division, National Institute of Biotechnology, Savar, Dhaka, Bangladesh

*Corresponding    Author's   E-mail: 

† These authors have equal contribution

Received May 7, 2013; Accepted May 27, 2013


Achyranthes aspera L. (Apang locally) belongs to the family Amaranthaceae, is a highly significant medicinal herb found almost everywhere in Bangladesh. The herbs are produced by using traditional propagation and in vitro techniques. Micropropagation is a rapid propagation technique, but the greatest problem is contamination with fungi and bacteria. We analyzed the effects of Nistatin, Flugal, Bavistin, Ridomil gold, and HgCl2 on surface sterilization to obtain desirable asepsis. However, among the different sterelants, Flugal 3% for 10min followed by 0.1% HgClfor 2-3min was the most effective treatment when field-grown plants (node) were used as explants. At higher concentration, fungicides were showed maximum effects against contaminants although survival percentages were low. This is the first report of successful sterilization and reduced contamination of explants from naturally field grown A. aspera L. by different sterilizing agents.


Keywords: Achyranthes aspera L., surface sterilization, asepsis, sterilizing agents.


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