|International Research Journal of Biotechnology Vol. 4(5), pp.94-100, May 2013
Copyright © 2013 International Research Journals
Full Length Research Paper
An efficient plant regeneration protocol for Achyranthes aspera L.
1Monokesh Kumer Sen†, *1,2Md. Mehedi Hassan†, 3Shamima Nasrin, 1M. Abu Hena Mostofa Jamal, 1A. N. M. Mamun-Or-Rashid, 1,4Nirupam Biswas
1Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh
2Department of Microbiology and Genetics and Institute for Medical Science, Chonbuk National University Medical School, Jeonju 561-712, South Korea
3Scientific Officer, Plant Biotechnology Division, National Institute of Biotechnology, Savar, Dhaka, Bangladesh
4Department of Nutrition and Food Sciences, University of Huddersfield, United Kingdom
*Corresponding Author's E-mail: firstname.lastname@example.org
† These authors have equal contribution
Received May 12, 2013; Accepted May 27, 2013
Achyranthes aspera L. (Apang locally) belongs to the family Amaranthaceae, is a highly significant medicinal herb found almost everywhere in Bangladesh. Mass collection of the plant from natural habitats has led to its depletion. An attempt was taken to establish an efficient protocol for its mass propagation from field-grown plants (node). Diverse concentrations of phytohormones in single or in various combinations were used to obtain desirable plant shoot and root regeneration. Experimentally, the best shoot induction was observed on half strength MS medium supplemented with BAP 3.0mg/L. In vitro raised shoots were allowed to root on different strengths of MS medium fortified with IAA, IBA and NAA at different concentrations. Concerted efforts of 2.0mg/L IBA and 0.5mg/L NAA were enabled to induce 100% root initiation with higher root number and longest shoot (17.0±0.75cm) on full strength MS medium. Afterwards, regenerated shoots with well developed roots were successfully subjected to hardening process and were acclimatized. The survived plantlets showed 86.67% survival frequency without any morphological abnormalities. This protocol might be applied for large scale commercial cultivation of Achyranthes aspera L..
Keywords: Achyranthes aspera L., Mass propagation, Phytohormones, MS medium, shoot and root induction, acclimatization.