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Full Length
Research Paper
Effects of high doses of Gelsemium sempervirens L. on
GABA receptor and on the cellular and humoral immunity in
mice
Hassan Rammal*,
Rachid Soulimani
Neurotoxicologie Alimentaire et Bioactivié, UR AFPA,
Université Paul Verlaine de Metz-INPL-INRA, BP 4102, 57040
Metz, France.
*Corresponding author
E-mail:
hasanrammal@hotmail.com. Tel/Fax: 0033387378506.
Received 29 January,2009; Accepted 15 March, 2010
Abstract
In this study, our objective was first to evaluate the
anxiolytic-like effect of the acute intraperitoneally
administration of high doses (9 CH, 15 CH and 30 CH) of
Gelsemium sempervirens L. in mouse model of anxiety, the
light/dark choice test. Secondly, to evaluate the effect of
the acute intraperitoneally administration of these same
doses on the cellular and humoral immunity in mice with
contrasting level of anxiety evaluated also in the
light/dark choice test. Our results showed that all doses of
G. sempervirens, more particularly the 30 CH,
presented a significant anxiolytic-like effect comparable to
that obtained by the well known anxiolytic diazepam. On the
other hand, flumazenil (a benzodiazepine receptor
antagonist) significantly blocked the effects induced by all
doses of the homeopathic drug G. Sempervirens which
also exerted an important effect on the cellular
(granulocytes, monocytes, total lymphocytes, TCD4+,
TCD8+ and NK cells) and humoral (Ig A, E and G)
immunity.
Keywords:
Gelsemium sempervirens,
anxiolytic-like, cellular and humoral immunity, light/dark
choice test,
diazepam, flumazenil.
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